Spectrophotometric Determination for 10-8 M Levels of L-Ascorbic Acid Using the Diazotization of 5,10,15,20-Tetrakis(4-aminophenyl)porphine.

• KAWAKAMI Takanori

• HONDA Kohji

• IGARASHI Shukuro

書誌事項

タイトル別名

• Spectrophotometric Determination for 10

抄録

A water-soluble porphyrin having four aminophenyl groups 5, 10, 15, 20-tetrakis(4-aminophenyl)porphine (TAPP) diazotizes with nitrite ion and produces a large spectral change (ε : molar absorptivity, Δε=ε_sample-ε_blank=3.0×10⁵M^-1cm^-1, 434nm). The coexistence of L-ascorbic acid (AsA) strongly interferes with this reaction. Therefore, a highly sensitive spectrophotometric determination of AsA was developed using this effect. For the determination of trace amounts of AsA, the calibration curve was linear in the concentration range of 8.0×10^-8M-5.0×10^-7M (14-88ppb). The detection limit (3σ) was 1.0×10^-8M (1.76ppb), and the relative standard deviation was 1.86% (2.0×10^-7M (35ppb) AsA, 6 determinations). This method is more than 20 times highly-sensitive than the indophenol method as standard spectrophotometry for AsA. This method has been applied to the deternation of AsA in soft drinks and drugs.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 45 (8), 1376-1378, 1997

  公益社団法人 日本薬学会

キーワード

• water-soluble porphyrin
• L-ascorbic acid
• diazotization
• soft drink
• drug
• spectrophotometric determination

詳細情報

• CRID: 1390001204160254720
• NII論文ID: 130003947341
• NII論文ID: 110003616675
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.45.1376
• ISSN: 13475223
• ISSN: 00092363
• NDL書誌ID: 4287819
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4287819
• Web Site: http://www.jstage.jst.go.jp/article/cpb1958/45/8/45_8_1376/_pdf
• 本文言語コード: en
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