Optimum Conditions for the ^<13>C-Phenylalanine Breath Test

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Abstract

We have conducted optimizaiton studies to develop a superior <SUP>13</SUP>C-phenylalanine breath test for the diagnosis of liver disease. First, we examined the optimum <SUP>13</SUP>C-labeling position in phenylalanine for use in a breath test based on infrared spectroscopic detection of <SUP>13</SUP>CO<SUB>2</SUB> in exhaled air. L-[1-<SUP>13</SUP>C]Phenylalanine gave the best result. Next, a suitable dosage to give a short peak time (the time expressed in minutes as which <SUP>13</SUP>CO<SUB>2</SUB> excretion is maximal) after administration was determined. The <SUP>13</SUP>CO<SUB>2</SUB>/<SUP>12</SUP>CO<SUB>2</SUB> ratio in exhaled air after administration of 100 mg/body of L-[1-<SUP>13</SUP>C]phenylalanine peaked sharply at 15 min. We also examined the effect of food on the hepatic metabolism of L-[1-<SUP>13</SUP>C]phenylalanine. We found that a fasting period of over 7 h before the test resulted in a higher <SUP>13</SUP>CO<SUB>2</SUB> peak excretion. The peak appeared sooner than that in the <SUP>13</SUP>C-phenacetin breath test and, therefore, the <SUP>13</SUP>C-phenylalanine breath test appears preferable for the rapid evaluation of hepatic function.

Journal

  • Chemical and Pharmaceutical Bulletin

    Chemical and Pharmaceutical Bulletin 46(8), 1330-1332, 1998-08-15

    The Pharmaceutical Society of Japan

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Codes

  • NII Article ID (NAID)
    110003617031
  • NII NACSIS-CAT ID (NCID)
    AA00602100
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00092363
  • NDL Article ID
    4540763
  • NDL Source Classification
    ZS51(科学技術--薬学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No.
    Z53-D167
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE 
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