Protective Effects of Baicalein against Cell Damage by Reactive Oxygen Species.

• GAO Dayuan

• TAWA Riichi

• MASAKI Hitoshi

• OKANO Yuri

• SAKURAI Hiromu

書誌事項

タイトル別名

• Protective Effects of Baicalein against

抄録

Baicalein (5, 6, 7-trihydroxy-2-phenyl-4H-1-benzopyran-4-one), a naturally occurring flavonoid, was found to prevent human dermal fibroblast cell damage induced by reactive oxygen species such as hydrogen peroxide (H₂O₂), tert-butyl hydroperoxide (BuOOH) and superoxide anions (·O^-₂) in a concentration-dependent manner, and was more effective than the iron chelator, deferoxamine, hydroxyl radical (·OH) scavengers such as dimethyl sulfoxide (DMSO) and ethanol (EtOH), the lipid peroxidation chain blocker, α-tocopherol (Vit. E) and the xanthine oxidase inhibitor, allopurinol. To probe the mechanism of cell defense, the reaction of baicalein with oxygen free radicals was investigated using electron spin resonance (ESR) spectrometry. Baicalein decreased the signal intensities due to the 5, 5-dimethyl-1-pyrroline-N-oxide (DMPO) spin adducts of ·OH, ·O^-₂ and tert-butyl peroxyl (BUOO·) radicals in a concentration-dependent manner. The IC₅₀ values, which are the 50% inhibition concentrations of baicalein for the free radicals, were 10, 45 and 310 μM, respectively. These results suggested that baicalein possesses free radical scavenging ability which prevents the fibroblast damage induced by these free radical species.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 46 (9), 1383-1387, 1998

  公益社団法人 日本薬学会

キーワード

• baicalein
• human dermal fibroblast
• active oxygen scavenging
• ESR
• spin-trapping method

詳細情報

• CRID: 1390001204164367872
• NII論文ID: 130003773859
• NII論文ID: 110003617043
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.46.1383
• COI: 1:CAS:528:DyaK1cXmtlCru7w%3D
• ISSN: 13475223
• ISSN: 00092363
• ISSN: http://id.crossref.org/issn/00092363
• NDL書誌ID: 4573184
• PubMed: 9775434
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4573184
• Web Site: http://www.jstage.jst.go.jp/article/cpb1958/46/9/46_9_1383/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1999003838
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可