Purification and partial characterization of the gastric ulcer inhibitory substance from culture filtrate of Bacillus subtilis H.
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The inhibitory substance against gastric ulceration in pylorus-ligated rats was purified through extraction with 5% acetic acid-50% ethanol, fractionation with ethanol, isoelectric precipitation, DEAE-cellulose column chromatography, and gel filtration on Sephadex G-100 column from isoelectric precipitate if the 72 hr culture filtrate of Bacillus subtilis H IAM 1521, and it was named gastric ulcer inhibitory substance (GUIS). This substance reduced ulceration in pylorus-ligated rats by 84.2% at the dose of 5.0mg/kg, it also significantly repressed aspirin-induced gastric lesions under pylorus ligation at the same dose, but its activity was weak in stress-induced ulceration. It markedly decreased gastric juice volume, acidity, and peptic activity in pylorus-ligated rats when administered intraperitoneally at 5.0 mg/kg. This substance was a glycoprotein which showed homogenous patterns in various kinds of electrophoresis, and its isoelectric point was pH 4.5.
- Chemical and Pharmaceutical Bulletin
Chemical and Pharmaceutical Bulletin 25(10), 2770-2774, 1977
The Pharmaceutical Society of Japan