Preparation of Fluorescence-Labeled and Cross-Linked Subtilisin

• 谷沢 和隆

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 真野 勉

  Faculty of Pharmaceutical Sciences, Hokkaido University

• 金岡 祐一

  Faculty of Pharmaceutical Sciences, Hokkaido University

抄録

Streptomyces subtilisin inhibitor (SSI) is a protein characterized by both its potent inhibitory activity toward subtilisin and its structure, composed of two homologous subunits. It binds two molecules of subtilisin to form a tetrameric complex.Intermolecularly cross-linked subtilisin is expected to form a polymeric complex with SSI. This could provide a useful model of protein-protein associatin. Therefore, Preparation of fluorescence-labeled and cross-linked subtilisin was carried out.

収録刊行物

• Chemical & pharmaceutical bulletin

  Chemical & pharmaceutical bulletin 38 (2), 464-466, 1990-02-25

  公益社団法人日本薬学会

キーワード

• subtilisin
• chemical modification
• cross-linking
• fluorescence label
• protein-protein interaction
• 2-aminothiolane
• disulfide exchange reaction
• subtilisin inhibitor

詳細情報

• CRID: 1572261552368011392
• NII論文ID: 110003628348
• NII書誌ID: AA00602100
• ISSN: 00092363
• 本文言語コード: en
• データソース種別:
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