NEW METHOD OF DETECTING NITRIC OXIDE PRODUCTION

  • KIKUCHI Kazuya
    Faculty of Pharmaceutical Sciences Faculty of Medicine, University of Tokyo
  • HAYAKAWA Hiroshi
    Second Department of Internal Medicine Faculty of Medicine, University of Tokyo
  • NAGANO Tetsuo
    Faculty of Pharmaceutical Sciences Faculty of Medicine, University of Tokyo
  • HIRATA Yasunobu
    Second Department of Internal Medicine Faculty of Medicine, University of Tokyo
  • SUGIMOTO Tsuneaki
    Second Department of Internal Medicine Faculty of Medicine, University of Tokyo
  • HIROBE Masaaki
    Faculty of Pharmaceutical Sciences Faculty of Medicine, University of Tokyo

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Abstract

Nitric oxide (NO) reacts with H_2O_2 to produce a rise in potent luminol-dependent chemiluminescence under neutral pH conditions. We applied this reaction to the continuous detection of NO released from the rat isolated perfused kidney with simultaneous pressure changes. Acetylcholine increased NO release dose-dependently with the pressure reduction. An NO synthesis inhibitor, N^G-monomethyl-L-arginine, abolished the release. This inhibition was reversed by the addition of L-arginine.

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Details 詳細情報について

  • CRID
    1572261552254058496
  • NII Article ID
    110003630194
  • NII Book ID
    AA00602100
  • ISSN
    00092363
  • Text Lang
    en
  • Data Source
    • CiNii Articles

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