The use of a new silylating agent for analysis of catecholamines by gas chromatography-mass spectrometry.

• 宮崎 浩

• 石橋 正兀

• 山下 幸和

• 薬師寺 晟

抄録

3-Methoxytyramine, dopamine, norepinephrine, epinephrine and 6-hydroxydopamine were derivatized to their N-trifluoroacetyl-O-dimethyl-n-propylsilyl ether or 2'-O-methyl-N-trifluoroacetyl-O-dimethyl-n-propylsilyl ether derivatives by treatment with trifluoroacetic anhydride, methanol and then dimethyl-n-propylsilyl imidazole. These derivatives could be separated completely by gas chromatography (GC) on a non-polar liquid stationary phase such as OV-101. In addition, these derivatives were very stable in benzene and ethyl acetate, and showed excellent stability during silica gel column chromatography in comparison with the corresponding trimethylsilyl ether derivatives. In GC-mass spectrometry (MS) of these derivatives, ammonia chemical ionization (CI)-MS provided the ion [M+NH₄]⁺ as the base peak or an intense peak. The detection limit of the dopamine derivative in the CI mode with ammonia was 2 pg with S/N at 2 : 1.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 29 (3), 796-803, 1981

  公益社団法人 日本薬学会

キーワード

• storage stability

詳細情報

• CRID: 1390001204163368064
• NII論文ID: 110003633734
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.29.796
• ISSN: 13475223
• ISSN: 00092363
• Web Site: http://www.jstage.jst.go.jp/article/cpb1958/29/3/29_3_796/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1982128712
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可