METABOLISM IN VITRO OF SULINDAC. SULFOXIDE-REDUCING ENZYME SYSTEMS IN GUINEA PIG LIVER

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Author(s)

Abstract

The sulfoxide reduction of sulindac (cis-5-fluoro-2-methyl-1-[p-(methylsulfinyl)-benzylidenyl] indene-3-acetic acid), an anti-inflammatory agent, was demonstrated in vitro by cell-free preparations of guinea pig liver. The sulfoxide reductase activity was located in both fractions of microsomes and 105000×g supernatant. The microsomal reductase was NADPH-or NADH-dependent, and required the factor present in the soluble fraction for its activity. The factor was heat-labile and non-dialyzable. Furthermore, a pattial purification of the microsomal NADPH-cytochrome c reductase resulted in a paralleled increase of the sulfoxide reductase activity. The present observations suggest that the soluble factor described above functions as an electron transfer component coupled with NADPH-cytochrome c reductase.

Journal

  • Journal of pharmacobio-dynamics

    Journal of pharmacobio-dynamics 3(6), 290-298, 1980-06

    The Pharmaceutical Society of Japan

Codes

  • NII Article ID (NAID)
    110003635897
  • NII NACSIS-CAT ID (NCID)
    AA00704585
  • Text Lang
    ENG
  • ISSN
    0386846X
  • Data Source
    NII-ELS 
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