METABOLISM IN VITRO OF SULINDAC. SULFOXIDE-REDUCING ENZYME SYSTEMS IN GUINEA PIG LIVER

• KITAMURA SHIGEYUKI

  Faculty of Pharmaceutical Sciences, Kyushu University (Present address)Research Laboratories. Nippon Merck-Banvu Co., Ltd.

• TATSUMI KIYOSHI

  Faculty of Pharmaceutical Sciences, Kyushu University

• YOSHIMURA HIDETOSHI

  Faculty of Pharmaceutical Sciences, Kyushu University

抄録

The sulfoxide reduction of sulindac (cis-5-fluoro-2-methyl-1-[p-(methylsulfinyl)-benzylidenyl] indene-3-acetic acid), an anti-inflammatory agent, was demonstrated in vitro by cell-free preparations of guinea pig liver. The sulfoxide reductase activity was located in both fractions of microsomes and 105000×g supernatant. The microsomal reductase was NADPH-or NADH-dependent, and required the factor present in the soluble fraction for its activity. The factor was heat-labile and non-dialyzable. Furthermore, a pattial purification of the microsomal NADPH-cytochrome c reductase resulted in a paralleled increase of the sulfoxide reductase activity. The present observations suggest that the soluble factor described above functions as an electron transfer component coupled with NADPH-cytochrome c reductase.

収録刊行物

• Journal of pharmacobio-dynamics

  Journal of pharmacobio-dynamics 3 (6), 290-298, 1980-06

  公益社団法人日本薬学会

キーワード

• metabolism in vitro
• sulindac
• guinea pig liver
• microsomes
• 105000×g supernatant
• sulfoxide reductase
• NADPH-cytochrome c reductase
• soluble factor

詳細情報

• CRID: 1570291227417328640
• NII論文ID: 110003635897
• NII書誌ID: AA00704585
• ISSN: 0386846X
• 本文言語コード: en
• データソース種別:
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