Molecular Cloning and Functional Expression of cDNAs Encoding Oxidosqualene Cyclases from Costus speciosus

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Abstract

<i>Costus speciosus</i> produces a large quantity of steroidal glycosides derived from the sole aglycone, diosgenin. Cycloartenol, a product of oxidosqualene cyclase (OSC), is postulated to be a common intermediate for phytosterols of primary metabolism and diosgenin of secondary metabolism, possibly providing a metabolic branch point. Two cDNAs, <i>CSOSC1</i> and <i>CSOSC2</i>, were cloned from <i>C. speciosus</i> by RT-PCR and cDNA library screening. Both cDNAs encode 759 amino acids with high mutual identity (74%), resembling (>55% identity) the known OSCs. Phylogenetic tree analysis indicated that the gene products occupy distinct positions from those of cycloartenol synthases (CASs) and triterpene synthases from dicotyledonous plants. By functional expression in yeast, <i>CSOSC1</i> and <i>CSOSC2</i> were proved to encode a CAS and a multifunctional triterpene synthase, respectively. The present result is the first demonstration of the functional expression of OSCs from monocotyledonous plants.

Journal

  • Biological and Pharmaceutical Bulletin

    Biological and Pharmaceutical Bulletin 25(4), 477-482, 2002-04-01

    The Pharmaceutical Society of Japan

References:  36

Cited by:  2

Codes

  • NII Article ID (NAID)
    110003638806
  • NII NACSIS-CAT ID (NCID)
    AA10885497
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09186158
  • NDL Article ID
    6120925
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-V41
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE 
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