Improved Nitric Oxide Detection Using 2,3-Diaminonaphthalene and Its Applicatio to the Evaluation of Novel Nitric Oxide Synthase Inhibitors

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Author(s)

Abstract

A specific and sensitive detection method for nitric oxide (NO) in living cells and tissue culture systems is required in the search for novel NO synthase (NOS) inhibitors. We have improved a fluorometric determination with 2, 3-diaminonaphthalene (DAN) by the addition of 2-phenyl-4, 4, 5, 5-tetramehylimidazoline-3-oxide-1-oxyl (PTIO) as an oxidant to form NO<SUB>2</SUB> from NO. This method is 3 times more sensitive than that without PTIO, and is suitable for examining the NOS-inhibitory activity of large numbers of test compounds using a 96-well micropolate reader. The improved method was applied to N-monomethyl-L-arginine (L-NMMA) as a known inhibitor and the derivatives of 2-phenyl-1, 2-benzisoselenazol-3(2H)-one as teat compounds in order to investigate the effect of these compounds on NO production from activated rat aortic smooth muscle cells. The results obtained indicate that this method is suitable for the rapid assay of large numbers of test compounds.

Journal

  • Biological and Pharmaceutical Bulletin

    Biological and Pharmaceutical Bulletin 21(12), 1247-1250, 1998-12-01

    The Pharmaceutical Society of Japan

References:  15

Cited by:  4

Codes

  • NII Article ID (NAID)
    110003639277
  • NII NACSIS-CAT ID (NCID)
    AA10885497
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09186158
  • NDL Article ID
    4626319
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-V41
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE 
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