Properties of Cationic Liposomes Composed of Cationic Lipid YKS-220 Having an Ester Linkage: Adequate Stability, High Transfection Efficiency, and Low Cytotoxicity.
Bibliographic Information
- Other Title
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- Properties of Cationic Liposomes Composed of Cationic Lipid YKS 220
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Abstract
Cationic lipid N-[3-[2-(1, 3-dioleoyloxy)propoxy-carbonyl]propyl]-N, N, N-trimethylammonium iodide (YKS-220) having a symmetrical and biodegradable structure was employed for the preparation of cationic liposomes with dioleoylphosphatidylethanolamine (DOPE). The stability, transfection activity in several cell lines and cytotoxicity of YKS-220 cationic liposomes were studied. It was found the YKS-220 cationic liposomes were very stable and their transfection activity remained even after storage at 4°C for 12 months. The transfection activity of these liposomes was assayed using CHO, COS, and HepG2 cells and found to be comparable with, or better than, that of other cationic liposomes, such as N-[1-(2, 3-dioleoyloxy)propyl]-N, N, N-trimethylammonium methylsulfate (DOTAP) liposome, N-[1-(2, 3-dioleyloxy)propyl]-N, N, N-trimethylammonium chloride (DOTMA) liposome (Lipofectin[○!R]), and 2, 3-dioleyloxy-N-[2-(sperminecarboxamido)ethyl]-N, N-dimethyl-1-propanaminium trifluoroacetate (DOSPA) liposome (LipofectAMINETM). n addition, the cytotoxicity of YKS-220 cationic liposomes was far lower than that of other cationic liposomes.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 22 (2), 187-190, 1999
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679603799296
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- NII Article ID
- 110003639632
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- NII Book ID
- AA10885497
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- COI
- 1:CAS:528:DyaK1MXhsFWjtbk%3D
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 4658806
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- PubMed
- 10077439
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed