Molecular Cloning and Characterization of Two cDNAs for Glycyrrhiza glabra Squalene Synthase.

• HAYASHI Hiroaki

• HIROTA Akiko

• HIRAOKA Noboru

• IKESHIRO Yasumasa

抄録

Two cDNAs (GgSQS1 and GgSQS2) encoding squalene synthase have been isolated from licorice, Glycyrrhiza glabra L., and characterized. The deduced amino acid sequence of GgSQS1 was 88%, 81%, 78%, 45-44%, and 45-41% identical to those of GgSQS2, Nicotiana, Arabidopsis, mammal and yeast squalene synthases, respectively. Squalene synthase activity was found in the cell-free extracts of Escherichia coli transformed with the recombinant plasmids for GgSQS1 and GgSQS2, respectively. Genomic Southern blot hybridization indicated that there are three squalene synthase genes in the licorice genome. Northern blot analysis showed that GgSQS2 mRNA is mainly expressed during the exponential growth phase of the cultured licorice cells.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 22 (9), 947-950, 1999

  公益社団法人 日本薬学会

キーワード

• Glycyrrhiza glabra L.
• Leguminosae
• squalene synthase
• biosynthesis
• tritepenoid
• cDNA

詳細情報

• CRID: 1390001204623113728
• NII論文ID: 110003639794
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.22.947
• COI: 1:CAS:528:DyaK1MXmtFCmtro%3D
• ISSN: 13475215
• ISSN: 09186158
• ISSN: http://id.crossref.org/issn/09186158
• NDL書誌ID: 4856815
• PubMed: 10513618
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4856815
• Web Site: http://www.jstage.jst.go.jp/article/bpb1993/22/9/22_9_947/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可