Biochemical Characterization of 60S Acidic Ribosomal P Proteins from Porcine Liver and the Inhibition of Their Immunocomplex Formation with Sera from Systemic Lupus Errythematosus(SLE) Patients by Glycyrrhizin in Vitro.

• MAEKAWA Toshiro

• KOSUGE Seiji

• KARINO Atsushi

• OKANO Tetsuro

• ITO Junko

• MUNAKATA Hiroshi

• OHTSUKI Kenzo

Bibliographic Information

Other Title

• Biochemical Characterization of 60S Acidic Ribosomal P Proteins from Porcine Liver and the Inhibition of Their Immunocomplex Formation with Sera from Systemic Lupus Erythematosus(SLE)Patients by Glycyrrhizin in Vitro

Abstract

The three casein kinase II (CK-II) phosphate acceptors (p35, p17 and p15) in the Superdex CK-II fraction prepared from a 1.5 M NaCl extract of porcine liver were selectively purified by glycyrrhizin (GL)-affinity column chromatography (HPLC) as a heterocomplex associated with CK-II. Determination of the N-terminal amino acid sequences and immunological tests confirmed that these three CK-II phosphate acceptors belong to the family of 60S acidic ribosomal proteins (P0, P1 and P2). Three polyphenol-containing anti-oxidant compounds [catechin, epigallocatechin gallate (EGCG) and quercetin] inhibited CK-II activity (phosphorylation of these ribosomal P proteins) in a dose-dependent manner in vitro. Quercetin (ID₅₀n=approx. 50 nM) was found to be an effective CK-II inhibitor. In contrast, CK-II activity was significantly stimulated by lower doses (0.3-3 μM) of GL, but was inhibited at high doses above 30 μM. As expected, GL at high doses above 200 μM inhibited the immunocomplex formation of 60S acidic ribosomal P proteins with their specific antibodies in the sera from patients with systemic lupus erythematosus (SLE). These results suggest that (i) a GL-affinity column is useful for effective purification of 60S acidic ribosomal P proteins from various mammalian cells as a heterocomplex associated with CK-II; and (ii) a relative high dose of GL may prevent the immunocomplex formation of 60S acidic ribosomal P proteins with their specific antibodies in the sera of SLE patients.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 23 (1), 27-32, 2000

  The Pharmaceutical Society of Japan

Keywords

• 60S acidic ribosomal P protein
• casein kinase II
• casein kinase II inhibitor
• glycyrrhizin
• glycyrrhizin-binding protein
• anti-oxidant compound

Details

• CRID: 1390282679603585664
• NII Article ID: 110003639818
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.23.27
• COI: 1:CAS:528:DC%2BD3cXmvFWrtg%3D%3D
• ISSN: 13475215
• ISSN: 09186158
• ISSN: http://id.crossref.org/issn/09186158
• NDL BIB ID: 4955617
• PubMed: 10706406
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4955617
• Web Site: http://www.jstage.jst.go.jp/article/bpb1993/23/1/23_1_27/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2000121552
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed