Evaluation of α-Glucosidase Inhibition by Using an Immobilized Assay System

• OKI Tomoyuki

• MATSUI Toshiro

• MATSUMOTO Kiyoshi

書誌事項

タイトル別名

• Evaluation of .ALPHA.-Glucosidase Inhibition by Using an Immobilized Assay System.
• Evaluation of アルファ Glucosidase Inhibition by Using an Immobilized Assay System

抄録

The inhibitory effects of natural and synthetic inhibitors on the intestinal membrane-bound hydrolase, α-glucosidase (AGH), were evaluated by using an immobilized cyanogen bromide-activated Sepharose 4B support. Immobilized AGH (iAGH) inhibition study by synthetic inhibitors (acarbose and voglibose) revealed that the magnitude of inhibition differed from that in the free AGH (fAGH) study : IC₅₀ value of acarbose in iAGH-maltase assay system, 340-430 nM; fAGH, 11 nM. iAGH-maltase inhibition by both inhibitors was influenced by blocking reagents with different functional groups (COOH, OH, CH₃, and NH₂ groups). On the other hand, significant iAGH-sucrase inhibitory activity was observed only when using the negatively charged support induced by 0.1 M β-alanine. The K_m values obtained in the iAGH assay system were similar to those from the fAGH method. With natural inhibitors, the iAGH-sucrase inhibitory activity of D-xylose, with in vivo glucose suppression, increased twice compared to that in fAGH. Green tea extract gave almost the same inhibition for both AGH assay systems.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 23 (9), 1084-1087, 2000

  公益社団法人 日本薬学会

キーワード

• α-glucosidase
• immobilization
• inhibition
• noninsulin-dependent diabetes mellitus

詳細情報

• CRID: 1390001204627767040
• NII論文ID: 110003640128
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.23.1084
• COI: 1:CAS:528:DC%2BD3cXmsVektLs%3D
• ISSN: 13475215
• ISSN: 09186158
• ISSN: http://id.crossref.org/issn/09186158
• NDL書誌ID: 5507252
• PubMed: 10993209
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5507252
• Web Site: http://www.jstage.jst.go.jp/article/bpb1993/23/9/23_9_1084/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2001023585
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可