Antiviral Activity of Plant Flavonoid, 5,7,4'-Trihydroxy-8-methoxyflavone, from the Roots of Scutellaria Baicalensis against Influenza A (H3N2) and B Viruses

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We investigated effects of isoscutellarein-8-methylether (5, 7, 4'-trihydroxy-8-methoxyflavone, F36) from the roots of Scutellaria baicalensis on the single-cycle replication of mouse-adapted influenza viruses A/Guizhou/54/89 (H3N2 subtype) and B/Ibaraki/2/85 in Madin-Darby canine kidney (MDCK) cells. The agent suppressed replication of these viruses from 6 to 12h after incubation in a dose-dependent manner by 50% at 20μM and 90% at 40μM, respectively. F36 (50μM) reduced the release of B/Ibaraki virus in the medium by 90-93% when it was added to the MDCK cells at 0 to 4 h after incubation. The cell-associated virus determined by sialidase activity was also reduced by the treatment at 0 to 4 h. F36 (120μM) inhibited the low pH-dependent membrane fusion of both the viruses with the liposome containing mixed gangliosides from bovine brain. However, the agent little affected the bemagglutination and RNA-dependent RNA polymerase activities of these viruses in vitro. These results suggest that F36 inhibits the replication of A/Guizhou and B/Ibaraki viruses at least partly by inhibiting the fusion of viral envelopes with the endosome/lysosome membrane which occurs at the early stage of the virus infection cycle. F36 (0.5mg/kg) showed no antiviral activity against A/Guizhou and B/Ibaraki viruses in mice when administered intranasally 5 min prior to virus inoculation, whereas it significantly inhibited their proliferation in the mouse lung when administered intranasally 7 times (total 3.5mg/kg) from 18h before to 54h after virus infection.

収録刊行物

  • Biological & pharmaceutical bulletin

    Biological & pharmaceutical bulletin 18(2), 295-299, 1995-02-15

    公益社団法人日本薬学会

参考文献:  18件中 1-18件 を表示

被引用文献:  7件中 1-7件 を表示

各種コード

  • NII論文ID(NAID)
    110003640827
  • NII書誌ID(NCID)
    AA10885497
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09186158
  • データ提供元
    CJP書誌  CJP引用  NII-ELS  J-STAGE 
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