Inhibitory Effect of Magnosalin Derived from Flos magnoliae on Tube Formation of Rat Vascular Endothelial Cells during the Angiogenic Process.

• KOBAYASHI Shinjiro

• KIMURA Ikuko

• KIMURA Masayasu

抄録

An aqueous water extract of Flos magnoliae, a Japanese Sino-medicine, inhibits angiogenesis in adjuvant-induced mouse pouch granuloma. Magnosalin (MSA) and magnoshinin(MSI), neolignans isolated from magnolia, have a crucial role in the anti-angiogenic effect of magnolia (Kimura et al., Int. Arch. Allergy Appl. Immunol., 93, 365 (1990); Phytother. Res., 6, 209 (1992)). We investigated the effects of these neolignans on tube formation of endothelial cells (EC) cultured in type I collagen gel during the angiogenic process. MSA (0.1-10 μM), MSI (0.23-7 μM) and corticosterone (CS : 0.3-30 μM) inhibited fetal bovine serum (FBS)-stimulated tube formation in a concentration-dependent manner. Their 30% inhibitory concentration (IC₃₀, 95% confidence limits) values were 0.51 (0.20-1.27) for MSA, 8.14 (2.48-26.7) for MSI and 3.65 μM (2.47-5.40) for CS, respectively. MSA and MSI (1-3 μM) also inhibited interleukin (IL)-1α-stimulated tube formation in a concentration-dependent manner. Their IC₅₀ values (95% confidence limits) were 1.22 (1.01-1.47) for MSA and 0.74 μM (0.24-2.31) for MSI against a submaximal concentration (69 pM) of IL-1α-stimulated tube formation. Their inhibitory effects on the action of IL-1α were non-competitive. These results demonstrate that MSA inhibited FBS-stimulated tube formation with a greater potency than MSI. The inhibitory effect of MSA on the action of FBS differed from that on the action of IL-1α.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 19 (10), 1304-1306, 1996

  公益社団法人 日本薬学会

キーワード

• magnosalin
• magnoshinin
• anti-tube formation
• anti-IL-1α activity
• anti-angiogenesis

詳細情報

• CRID: 1390001204622190208
• NII論文ID: 110003641092
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.19.1304
• COI: 1:CAS:528:DyaK28XmsVGiu70%3D
• ISSN: 13475215
• ISSN: 09186158
• ISSN: http://id.crossref.org/issn/09186158
• PubMed: 8913501
• Web Site: http://www.jstage.jst.go.jp/article/bpb1993/19/10/19_10_1304/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1997109914
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可