Acceleration of Glycolysis in Erythrocytes by the Antidiabetic Agent M16209

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Author(s)

    • HASHIMOTO Koji
    • Department of Pharmacology, Fuji Central Research Laboratory, Mochida Pharmaceutical Co., Ltd.
    • MURAKAMI Nobuya
    • Department of Pharmacology, Fuji Central Research Laboratory, Mochida Pharmaceutical Co., Ltd.
    • OHTA Masahiko
    • Department of Pharmacology, Fuji Central Research Laboratory, Mochida Pharmaceutical Co., Ltd.
    • KATO Katsuaki
    • Department of Pharmacology, Fuji Central Research Laboratory, Mochida Pharmaceutical Co., Ltd.
    • MIZOTA Masahiro
    • Department of Pharmacology, Fuji Central Research Laboratory, Mochida Pharmaceutical Co., Ltd.
    • MIWA Ichitomo
    • Department of Pathobiochemistry, Faculty of Pharmacy, Meijo University
    • OKUDA Jun
    • Department of Clinical Biochemistry, Faculty of Pharmacy, Meijo University

Abstract

The effects of M16209 (1-(3-bromobenzofuran-2-ylsulfonyl)hydantoin), an antidiabetic agent and aldose reductase inhibitor, on glycolysis were studied in rat and human erythrocytes in vitro. M16209 increased lactate production from glucose when incubated with rat and human erythrocytes, and also increased glucose consumption in rat erythrocytes. The rates of production of lactate in rat erythrocytes treated with M16209 at 10, 25 and 50 μM were 113, 118 and 123%, respectively, of those in vehicle treated cells. Sorbinil (aldose reductase inhibitor), tolbutamide (sulfonylurea), and buformine (biguanide) did not increase lactate production in rat erythrocytes when tested at 50 μM. On the other hand, M16209 did not affect lactate production from D-glyceraldehyde in rat erythrocytes. At 100 μM the agent decreased both glucose-6-phosphate and fructose-6-phosphate in rat erythrocytes, and increased fructose-1, 6-bisphosphate; at 10 μM it also increased 6-phosphofructokinase activity in rat hemolysates. These findings suggest that M16209 accelerates glycolysis in erythrocytes via activation of 6-phosphofructokinase.

Journal

  • Biological and Pharmaceutical Bulletin

    Biological and Pharmaceutical Bulletin 19(6), 809-813, 1996-06-15

    The Pharmaceutical Society of Japan

References:  19

Codes

  • NII Article ID (NAID)
    110003641267
  • NII NACSIS-CAT ID (NCID)
    AA10885497
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09186158
  • Data Source
    CJP  NII-ELS  J-STAGE 
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