Acceleration of Glycolysis in Erythrocytes by the Antidiabetic Agent M16209.

• HASHIMOTO Koji

• MURAKAMI Nobuya

• OHTA Masahiko

• KATO Katsuaki

• MIZOTA Masahiro

• MIWA Ichitomo

• OKUDA Jun

抄録

The effects of M16209 (1-(3-bromobenzofuran-2-ylsulfonyl)hydantoin), an antidiabetic agent and aldose reductase inhibitor, on glycolysis were studied in rat and human erythrocytes in vitro. M16209 increased lactate production from glucose when incubated with rat and human erythrocytes, and also increased glucose consumption in rat erythrocytes. The rates of production of lactate in rat erythrocytes treated with M16209 at 10, 25 and 50 μM were 113, 118 and 123%, respectively, of those in vehicle treated cells. Sorbinil (aldose reductase inhibitor), tolbutamide (sulfonylurea), and buformine (biguanide) did not increase lactate production in rat erythrocytes when tested at 50 μM. On the other hand, M16209 did not affect lactate production from D-glyceraldehyde in rat erythrocytes. At 100 μM the agent decreased both glucose-6-phosphate and fructose-6-phosphate in rat erythrocytes, and increased fructose-1, 6-bisphosphate; at 10 μM it also increased 6-phosphofructokinase activity in rat hemolysates. These findings suggest that M16209 accelerates glycolysis in erythrocytes via activation of 6-phosphofructokinase.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 19 (6), 809-813, 1996

  公益社団法人 日本薬学会

キーワード

• 1-(3-bromobenzofuran-2-ylsulfonyl)hydantoin (M16209)
• glycolysis
• erythrocyte
• phosphofructokinase
• D-glyceraldehyde
• lactate production

詳細情報

• CRID: 1390001204623121152
• NII論文ID: 110003641267
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.19.809
• ISSN: 13475215
• ISSN: 09186158
• PubMed: 8799477
• Web Site: http://www.jstage.jst.go.jp/article/bpb1993/19/6/19_6_809/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1996221973
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可