In Vivo Testing System for Determining the Estrogenic Activity of Endocrine-Disrupting Chemicals(EDCs) in Goldfish (Carassius auratus).
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- Ishibashi Hiroshi
- Graduate School of Science and Technology, Nagasaki University
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- Tachibana Katsuyasu
- Graduate School of Science and Technology, Nagasaki University
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- Tsuchimoto Mutsuyosi
- Graduate School of Science and Technology, Nagasaki University
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- Soyano Kiyoshi
- Graduate School of Science and Technology, Nagasaki University
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- Ishibashi Yasuhiro
- Environmental Protection center, Nagasaki University
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- Nagae Masaki
- Faculty of Environmental Studies, Nagasaki University
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- Kohra Shinya
- Faculty of Environmental Studies, Nagasaki University
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- Takao Yuji
- Faculty of Environmental Studies, Nagasaki University
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- Tominaga Nobuaki
- Department of Chemical and Biological Engineering, Ariake National College of Technology
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- Arizono Koji
- Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto
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抄録
Vitellogenin (VTG) is believed to be an effective and sensitive biomarker to detect the effects of endocrine-disrupting chemicals (EDCs) on fish. An enzymelinked immunosorbent assay (ELISA) was developed in this study for the quantification of VTG in goldfish (Carassius auratus) blood plasma using a monoclonal antibody against carp lipovitellin which is known to cross-react with goldfish VTG, and a working range of 7.8 to 500 ng VTG/ml was established. A laboratory study involving the dosing of male goldfish for 28 days with a range of bisphenol-A (BPA) concentrations was conducted, and the established ELISA was used. There was no significant induction of VTG at the concentrations of 1 and 10 μg/l. Exposure to 100 μg/l and 1000 μg/l BPA for 28 days significantly elevated plasma VTG concentrations to 201 ± 90 μg/ml and 104552 ± 24920 μg/ml, respectively. To confirm the effect of temperature on VTG induction, male goldfish were exposed to the nominal concentration of 17β-es-tradiol (E2) 100 μg/l at 10° and 30°C for 10 days. Plasma VTG concentration in male goldfish increased more quickly in the fish exposed at 30°C than in those at 10°C. In addition, the plasma VTG concentration in the 30°C group (10463 ± 3268 μg/ml) was higher than that of the 10°C group (1.42 ± 1.6 μg/ml) after 1 day of exposure. Thus goldfish are useful for the investigation of EDCs under various conditions since they are relatively small in size, and the ELISA established in the present study will contribute to various types of research.
収録刊行物
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- JOURNAL OF HEALTH SCIENCE
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JOURNAL OF HEALTH SCIENCE 47 (2), 213-218, 2001
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679474724352
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- NII論文ID
- 110003641398
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- NII書誌ID
- AA11316464
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- COI
- 1:CAS:528:DC%2BD3MXivFektb8%3D
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- ISSN
- 13475207
- 13449702
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- HANDLE
- 10069/8334
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- NDL書誌ID
- 5727641
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可