Production and Application of Monoclonal Antibodies to Human Selenoprotein P.

• Saito Yoshiro

  Department of Hygienic Chemistry, Graduate School of Pharmaceutical Science, Hokkaido University

• Watanabe Yasuko

  Department of Hygienic Chemistry, Graduate School of Pharmaceutical Science, Hokkaido University

• Saito Eiji

  Second Department of Internal Medicine, Nihon University School of Medicine

• Honjoh Tsutomu

  Morinaga Institute of Biological Sciences

• Takahashi Kazuhiko

  Department of Hygienic Chemistry, Graduate School of Pharmaceutical Science, Hokkaido University

抄録

Selenoprotein P is a selenium-rich extracellular protein and is the major selenoprotein in plasma. Although the biological function of selenoprotein P has not been established, we have recently shown that selenoprotein P has phospholipid hydroperoxide glutathione peroxidase-like activity. To study the structure and function of selenoprotein P, we produced monoclonal antibodies against human selenoprotein P. Immunization of rats with purified selenoprotein P was followed by hybridization, cloning, and the establishment of eleven hybridomas producing specific antibodies against human selenoprotein P. With the addition of six kinds of insoluble rat anti-human selenoprotein P monoclonal antibodies to human plasma, the selenium concentration of the supernatant was decreased to 47% of the control. This suggests that selenoprotein P constituted 53% of total plasma selenium. Western blot analysis of the immunoprecipitate from human plasma showed that the antibodies specifically bound to a 69 kDa protein, representing selenoprotein P. Next, we developed an enzyme-linked immunosorbent assay for selenoprotein P using two monoclonal antibodies. The plasma concentration of selenoprotein P in 77 normal individuals was 5.3 ± 1.1 μg/ml. Because preferential depletion of selenoprotein P by low density lipoprotein (LDL)-apheresis has been reported, we next measured selenoprotein P concentration of plasma samples from patients before and after LDL-apheresis. We confirmed that the plasma concentration of selenoprotein P was decreased from 5.7 to 2.3 μg/ml by LDL-apheresis. This result shows that this assay provides a reliable means of measuring the content of selenoprotein P in plasma.

収録刊行物

• ＪＯＵＲＮＡＬ　ＯＦ　ＨＥＡＬＴＨ　ＳＣＩＥＮＣＥ

  ＪＯＵＲＮＡＬ　ＯＦ　ＨＥＡＬＴＨ　ＳＣＩＥＮＣＥ 47 (4), 346-352, 2001

  公益社団法人 日本薬学会

キーワード

• selenium
• enzyme-linked immunosorbent assay
• selenoprotein

詳細情報

• CRID: 1390001204499212288
• NII論文ID: 110003641415
• NII書誌ID: AA11316464
• DOI: 10.1248/jhs.47.346
• COI: 1:CAS:528:DC%2BD3MXlslynurY%3D
• ISSN: 13475207
• ISSN: 13449702
• NDL書誌ID: 5852381
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5852381
• Web Site: http://www.jstage.jst.go.jp/article/jhs/47/4/47_4_346/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2002049873
• 本文言語コード: en
• データソース種別:
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