Induction of Apoptosis with an Extract of Actinidia polygama Fruit in the Promyelocytic Leukemia Cell Line HL-60

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Author(s)

Abstract

Common small fruit produced naturally in Japan is attracted as healthy food. We studied on inhibitory activity of several small fruit extracts on growth of cancer cells to look for a candidate of healthy food or low toxic anti-cancer drugs. Among the fruit extracts examined, <i>Actinidia polygama</i> (Matatabi) extract had dose-dependent potent inhibitory activity against the growth of a promyelocytic leukemia cell line, HL-60, but was less effective on a colon cancer cell line, LS-174T, or normal human dermal fibroblast cells. We examined a part of the responsible mechanism of growth inhibition of HL-60 cells. Active agents in <i>A. polygama</i> fruit were heat-stable and small molecular weight of less than 1000. HL-60 cells incubated with an extract of <i>A. polygama</i> fruit induced apoptosis, which was detected by electrophoretic DNA fragmentation, morphological chromatin condensation and the TUNEL method with flow cytometry.<br>

Journal

  • Journal of Health Science

    Journal of Health Science 48(4), 303-309, 2002-08-01

    The Pharmaceutical Society of Japan

References:  17

Codes

  • NII Article ID (NAID)
    110003641472
  • NII NACSIS-CAT ID (NCID)
    AA11316464
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13449702
  • NDL Article ID
    6243000
  • NDL Source Classification
    ZS17(科学技術--医学--衛生学・公衆衛生)
  • NDL Call No.
    Z54-J464
  • Data Source
    CJP  NDL  NII-ELS  J-STAGE 
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