ゲル濾過を用いた食品添加物カラギーナン中の単糖, 二糖の定量

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タイトル別名
  • Determination of Mono-and Disaccharides in Carrageenans of Food Additives Using Gel Filtration

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The identification and determination of cabohydrates in low molecular fraction and determination of molecular weight of carrageenans in high molecular fraction of 5 carrageenans of food additives separated by Sephacryl S-300 column chromatography were studied. Lactose, glucose or sucrose was identified by thin-layer chromatography (TLC) as low molecular weight carbohydrates in carrageenan of food additives. By the phenol-sulfuric acid method by use of saccharide identified by TLC as a standard, the contents of mono-and disaccharides in carrageenans of food additives obtained from low molecular fraction separated by Sephacryl gel filtration, outer solution dialysed with cellophane tube and extraction with 80% methanol were found to be within the range of 20-60%. The molecular weight was more than 100000 for each of carrageenans in carrageenans of food additives by Sephacryl S-300 chromatography with well-characterized dextrans. Sulfate contents of carrageenans by the barium sulfate gravimetric method were found to be above 18%, except one to be 7.5%.

収録刊行物

  • 衛生化学

    衛生化学 31 (2), 137-140, 1985-04-30

    公益社団法人日本薬学会

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