ヒドロキシプロリンの改良定量法

• 永谷 康典

• 武藤 〓章

• 佐藤 宏

• 飯島 昌夫

書誌事項

タイトル別名

• An Improved Method for the Determination of Hydroxyproline
• ヒドロキシプロリン ノ カイリョウ テイリョウホウ

抄録

An improved method for the determination of hydroxyproline (Hyp) in animal tissues has been established by modifying Kivirikko's and KISO methods. This method consists of the oxidation of Hyp by the addition of chloramine T at 0°C for 2 h in 0.5 M borate buffer (pH 8.2) containing 1 M KCl. It was confirmed that Hyp was satisfactorily oxidized to an oxidation intermediate, which was stable throughout the process of the oxidation at 0°C for 2 h. The degree of color development with the colorimetric reagent in this method was 2-3 times higher than that in conventional methods, and the reproducibility was invariably good. The present method is very useful for the microanalytical determination (0.05-4 μg/tube) of Hyp.

収録刊行物

• 薬学雑誌

  薬学雑誌 106 (1), 41-46, 1986

  公益社団法人 日本薬学会

キーワード

• hydroxyproline
• collagen
• animal tissue
• determination
• colorimetry
• chloramine T

詳細情報

• CRID: 1390001206153827072
• NII論文ID: 110003648547
• NII書誌ID: AN00284903
• DOI: 10.1248/yakushi1947.106.1_41
• ISSN: 13475231
• ISSN: 00316903
• ISSN: http://id.crossref.org/issn/00316903
• NDL書誌ID: 3074316
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3074316
• Web Site: https://www.jstage.jst.go.jp/article/yakushi1947/106/1/106_1_41/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1986161395
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可