利水作用を有する生薬のウマ腎臓(Na<SUP>+</SUP>+K<SUP>+</SUP>)-Adenosine Triphosphataseに対する影響 The Effects of Crude Drugs Using Diuretic on Horse Kidney (Na<SUP>+</SUP>+K<SUP>+</SUP>)-Adenosine Triphosphatase

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Abstract

In the folk-medicine, several kinds of crude drugs are used as diuretics. Twenty three kinds of diuretic drugs were chosen, and examined for their effects on the horse kidney (Na<SUP>+</SUP>+K<SUP>+</SUP>)-adenosine triphosphatase (ATPase), which is an intrinsic enzyme of the plasma membrane and responsible for the active transport of Na<SUP>+</SUP> and K<SUP>+</SUP> across the membrane. Twenty one out of twenty three kinds of ethanol extracts of diuretic drugs inhibited the kidney (Na<SUP>+</SUP>+K<SUP>+</SUP>)-ATPase activity. The intensity of the inhibition of these drugs was compared by estimating the amounts of their ethanol extracts which inhibited the (Na<SUP>+</SUP>+K<SUP>+</SUP>)-ATPase activity by 50% (I<SUB>50</SUB>, μg/ml). Among these drugs, Atractylodis Lanceae Rhizoma ( ?? ??, I<SUB>50</SUB>=12.8) Atractylodis Rhizoma ( ?? ??, I<SUB>50</SUB>=15.2), Plantaginis Semen ( ?? ?? ??, I<SUB>50</SUB>=16.0), Plantaginis Herba ( ?? ?? ??, I<SUB>50</SUB>=16.0) and Alismatis Rhizoma ( ?? ??, I<SUB>50</SUB>=22.0), have strong inhibitory effects on the kidney (Na<SUP>+</SUP>+K<SUP>+</SUP>)-ATPase activity. The ethanol extracts of the rhizomes of Atractylodes lancea DE CANDOLLE ( ?? ?? ?? ?? ) and Atractylodis japonica KITAMURA ( ?? ?? ?? ) were examined with varying concentrations of ATP and ouabain. The mode of inhibition of these two extracts on the (Na<SUP>+</SUP>+K<SUP>+</SUP>)-ATPase activity appeared to be uncompetitive with respect to ATP as judged from Line-weaver-Burk plot. The ethanol extract of Atractylodes japonica KITAMURA decreased the I<SUB>50</SUB> for ouabain from 1.6×10<SUP>-7</SUP> to 7.0×10<SUP>-9</SUP>M, while that of Atractylodes lancea DE CANDOLLE did not change the I<SUB>50</SUB> for ouabain.

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 111(2), 138-145, 1991

    The Pharmaceutical Society of Japan

Cited by:  2

Codes

  • NII Article ID (NAID)
    110003649214
  • NII NACSIS-CAT ID (NCID)
    AN00284903
  • Text Lang
    UNK
  • Article Type
    Journal Article
  • ISSN
    0031-6903
  • NDL Article ID
    3711222
  • NDL Source Classification
    SD0(薬学)
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z19-411
  • Data Source
    CJPref  NDL  NII-ELS  J-STAGE 
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