書誌事項
- タイトル別名
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- Inhibitory Effect of Bithionol on NADH-Fumarate Reductase in Ascarides
- カイチュウ ノ NADH フマルサン レダクターゼ ニ タイスル ビチオノール
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抄録
To elucidate the mechanism of anthelmintic action of bithionol, the inhibitory effect of the drug on NADH-fumarate reductase (NADH-FR) of Ascaris lumbricoides suum was examined. NADH-FR, an enzyme of anaerobic carbohydrate metabolic pathway was solubilized from the mitochondria of the worm's muscle with deoxycholate, and then partially purified with the monoethanolamine-Sepharose 4B column chromatography. Rhodoquinone (RQ), which is required for the electron transfer from NADH to fumarate, was separated from the enzyme protein and phospholipids. Although the enzyme protein fraction eluted from the above column did not show NADH-FR activity, this enzyme was reactivated by the addition of purified RQ and phosphatidylcholine. The IC50 value of bithionol for reconstituted NADH-FR was 18±2μM. The inhibition type was competitive to RQ. Bithionol inhibited at most 30% NADH-ferricyanide reductase, which did not require RQ, even at high concentration of 150μM. These results suggest that the pharmacological action of bithionol, a phenolic anthelmintic, depends on the inhibition of the electron transport system by the competition with RQ.
収録刊行物
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- 薬学雑誌
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薬学雑誌 113 (9), 663-669, 1993
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282681131154944
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- NII論文ID
- 110003649433
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- NII書誌ID
- AN00284903
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- ISSN
- 13475231
- 00316903
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- NDL書誌ID
- 3844407
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- データソース種別
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- JaLC
- NDL
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