An ultramicro phosphorimetric assay for alkaline and acid phosphatase activities.

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A sensitive phosphorimetric method for the assay of alkaline and acid phosphatases in biological materials is described. p-Nitrophenol, formed enzymatically from the substrate p-nitrophenyl phosphate, is extracted with ether and then determined phosphorimetrically in a mixture of ether and ethanolic potassium hydroxide. The method is rapid and very sensitive, and thus requires as little as 0.5-5μl of human serum or 0.1-5μg of protein of rat tissue with a detection limit for p-nitrophenol formed of as little as 10 pmol.

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