An ultramicro phosphorimetric assay for alkaline and acid phosphatase activities.
この論文をさがす
抄録
A sensitive phosphorimetric method for the assay of alkaline and acid phosphatases in biological materials is described. p-Nitrophenol, formed enzymatically from the substrate p-nitrophenyl phosphate, is extracted with ether and then determined phosphorimetrically in a mixture of ether and ethanolic potassium hydroxide. The method is rapid and very sensitive, and thus requires as little as 0.5-5μl of human serum or 0.1-5μg of protein of rat tissue with a detection limit for p-nitrophenol formed of as little as 10 pmol.
収録刊行物
-
- CHEMICAL & PHARMACEUTICAL BULLETIN
-
CHEMICAL & PHARMACEUTICAL BULLETIN 28 (10), 3127-3130, 1980
公益社団法人 日本薬学会
- Tweet
キーワード
詳細情報
-
- CRID
- 1390001204162010752
-
- NII論文ID
- 110003662430
-
- NII書誌ID
- AA00602100
-
- ISSN
- 13475223
- 00092363
-
- PubMed
- 7448947
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可