Spectrofluorometric Determination of Uric Acid and Glucose by Use of Fe(III)-Thiacalix[4]arenetetrasulfonate as a Peroxidase Mimic
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- Odo Junichi
- Department of Biological Chemistry, Faculty of Science, Okayama University of Science
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- Shinmoto Eri
- Department of Biological Chemistry, Faculty of Science, Okayama University of Science
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- Shiozaki Atsushi
- Department of Biological Chemistry, Faculty of Science, Okayama University of Science
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- Hatae Yoko
- Department of Biological Chemistry, Faculty of Science, Okayama University of Science
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- Katayama Seiichi
- Department of Biological Chemistry, Faculty of Science, Okayama University of Science
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- Jiao Geng-sheng
- Department of Chemistry, Weinan Teachers College
書誌事項
- タイトル別名
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- Spectrofluorometric Determination of Uric Acid and Glucose by Use of Fe 3 Thiacalix 4 arenetetrasulfonate as a Peroxidase Mimic
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The peroxidase-like activity of the Fe3+ complex of thiacalix[4]arenetetrasulfonate on a modified anion-exchanger (Fe3+-TCAS[4]A-500) has been applied to the spectrofluorometric determination of uric acid and glucose in connection with uricase and glucoseoxidase (GOD), respectively. Uric acid and glucose were spectrofluorometrically determined by measuring the amounts of H2O2 produced through each catalytic reaction by uricase and GOD for uric acid and glucose in a sample solution, respectively. The calibration curves obtained by the Uricase-Fe3+-TCAS[4]A-500 and the GOD-Fe3+-TCAS[4]A-500 methods were linear from 0.5 to 5.0 μg of uric acid in a 1.0 ml sample solution and from 0.5 to 6.0 μg of glucose in a 0.5 ml sample solution, respectively. The methods using Fe3+-TCAS[4]A-500 were applied to the determination of uric acid and glucose in control sera. <br>
収録刊行物
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- JOURNAL OF HEALTH SCIENCE
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JOURNAL OF HEALTH SCIENCE 50 (6), 594-599, 2004
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679475483136
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- NII論文ID
- 110003666008
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- NII書誌ID
- AA11316464
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- COI
- 1:CAS:528:DC%2BD2cXhtFSltLbF
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- ISSN
- 13475207
- 13449702
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- NDL書誌ID
- 7172859
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可