Spectrofluorometric Determination of Uric Acid and Glucose by Use of Fe(III)-Thiacalix[4]arenetetrasulfonate as a Peroxidase Mimic

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  • Odo Junichi
    Department of Biological Chemistry, Faculty of Science, Okayama University of Science
  • Shinmoto Eri
    Department of Biological Chemistry, Faculty of Science, Okayama University of Science
  • Shiozaki Atsushi
    Department of Biological Chemistry, Faculty of Science, Okayama University of Science
  • Hatae Yoko
    Department of Biological Chemistry, Faculty of Science, Okayama University of Science
  • Katayama Seiichi
    Department of Biological Chemistry, Faculty of Science, Okayama University of Science
  • Jiao Geng-sheng
    Department of Chemistry, Weinan Teachers College

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  • Spectrofluorometric Determination of Uric Acid and Glucose by Use of Fe 3 Thiacalix 4 arenetetrasulfonate as a Peroxidase Mimic

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Abstract

The peroxidase-like activity of the Fe3+ complex of thiacalix[4]arenetetrasulfonate on a modified anion-exchanger (Fe3+-TCAS[4]A-500) has been applied to the spectrofluorometric determination of uric acid and glucose in connection with uricase and glucoseoxidase (GOD), respectively. Uric acid and glucose were spectrofluorometrically determined by measuring the amounts of H2O2 produced through each catalytic reaction by uricase and GOD for uric acid and glucose in a sample solution, respectively. The calibration curves obtained by the Uricase-Fe3+-TCAS[4]A-500 and the GOD-Fe3+-TCAS[4]A-500 methods were linear from 0.5 to 5.0 μg of uric acid in a 1.0 ml sample solution and from 0.5 to 6.0 μg of glucose in a 0.5 ml sample solution, respectively. The methods using Fe3+-TCAS[4]A-500 were applied to the determination of uric acid and glucose in control sera. <br>

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