Stimulation of Proteoglycan Release from Cultured Vascular Endothelial Cell Layers by Sodium Spirulan

• Kaji Toshiyuki

  Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University

• Shimada Satomi

  Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University

• Yamamoto Chika

  Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University

• Fujiwara Yasuyuki

  Department of Environmental Health, Faculty of Pharmaceutical Sciences, Hokuriku University

• Lee Jung-Bum

  Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• Hayashi Toshimitsu

  Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

抄録

Our previous study suggested that sodium spirulan (Na-SP) stimulated the release of proteoglycans from cultured vascular endothelial cell layers. In the present study, we characterized the stimulatory effect of Na-SP on endothelial proteoglycan release from the viewpoint of the Na-SP structure. The following results were obtained: Na-SP is a potent stimulator of endothelial proteoglycan release. The stimulatory effect of Na-SP disappears with desulfation or removal of sodium ion. Depolymerized Na-SPs (M_r ∼14700 or greater) also exhibits stimulatory effects as does Na-SP (M_r ∼220000). The stimulation occurs more potently in endothelial cells than in vascular smooth muscle cells. Therefore it is suggested that the stimulatory effect of Na-SP on proteoglycan release requires both the sulfate group and sodium ion, and the effect is achieved by an active structure in the molecule which interacts particularly with the endothelial cell extracellular matrix. <br>

収録刊行物

• ＪＯＵＲＮＡＬ　ＯＦ　ＨＥＡＬＴＨ　ＳＣＩＥＮＣＥ

  ＪＯＵＲＮＡＬ　ＯＦ　ＨＥＡＬＴＨ　ＳＣＩＥＮＣＥ 50 (6), 654-659, 2004

  公益社団法人 日本薬学会

キーワード

• sodium spirulan
• endothelial cell
• proteoglycan
• extracellular matrix
• vascular smooth muscle

詳細情報

• CRID: 1390282679475510016
• NII論文ID: 110003666017
• NII書誌ID: AA11316464
• DOI: 10.1248/jhs.50.654
• ISSN: 13475207
• ISSN: 13449702
• NDL書誌ID: 7172950
• Web Site: http://id.ndl.go.jp/bib/7172950
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7172950
• Web Site: http://www.jstage.jst.go.jp/article/jhs/50/6/50_6_654/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006178562
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可