The Potential Contribution of Phytoestrogens and Organochlorine Pesticides in an Experimental Fish Diet to Estrogenic Activity

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Author(s)

Abstract

Phytoestrogens and organochlorine pesticides in the diet of laboratory animals are a possible source of interference in bioassays that assess estrogenic activity. In the present study, we investigated the levels of dietary phytoestrogens, organochlorine pesticides and the estrogenic activity of various diets for an experimental fish and discuss the potential contribution of these substances to estrogenic activity, in comparison with those used in previous studies. After hydrolysis with <i>β</i>-glucuronidase, genistein and daidzein were detected in all of the diets, and there were no significant differences in the contents of these substances among present and previous investigations. In addition, organochlorine pesticides, such as hexachlorobenzene (HCB), <i>β</i>-benzene hexachloride (<i>β</i>-BHC), <i>γ</i>-BHC, <I>trans</I>-nonachlor, and/or endrin, were detected in most fish diets. All of these diets exhibited higher levels of activation of <i>β</i> estrogen receptors than with <i>α</i> estrogen receptors in an <I>in vitro</I> yeast-based bioassay. These results indicate that phytoestrogens, such as genistein and daidzein, were the main substances contributing to the estrogenic activity of the diet. Moreover, some diets may exert estrogenic activity in <I>in vivo</I> tests, indicating the necessity for more careful selection of the feeding diet and measurement of estrogenic substances when performing routine screening assays for endocrine-disrupting chemicals. <br>

Journal

  • Journal of Health Science

    Journal of Health Science 51(2), 212-219, 2005-04-01

    Pharmaceutical Society of Japan

References:  28

Cited by:  1

Codes

  • NII Article ID (NAID)
    110003666056
  • NII NACSIS-CAT ID (NCID)
    AA11316464
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13449702
  • NDL Article ID
    7317038
  • NDL Source Classification
    ZS17(科学技術--医学--衛生学・公衆衛生)
  • NDL Call No.
    Z54-J464
  • Data Source
    CJP  CJPref  NDL  NII-ELS  IR  J-STAGE 
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