Post-biotinylation of Photocrosslinking by Staudinger-Bertozzi Ligation of Preinstalled Alkylazide Tag

• Hashimoto Makoto

  Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine

• Hatanaka Yasumaru

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

抄録

Post-biotinylation of the alkyl azide derivative of trifluoromethyl phenyldiazirine (TPD) was elucidated to apply a photoaffinity biotinylation technique. A photo-modified polyvinilidene difluoride (PVDF) membrane was used as a photolabeled component and we introduced biotin by Staudinger–Bertozzi ligation. The 15 pmol amount of biotinylated reagent was still effective for the visualization of cross-linked product on the matrix. The results show the potential utility of alkyl azide carrying TPD derivatives in the application of photoaffinity biotinylation, which could be useful for the ligands with tight structural requirements.

収録刊行物

• ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ

  ＣＨＥＭＩＣＡＬ　＆　ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ　ＢＵＬＬＥＴＩＮ 53 (11), 1510-1512, 2005

  公益社団法人 日本薬学会

キーワード

• diazirine
• photoaffinity label
• chemical biology
• Staudinger reaction
• avidin-biotin

詳細情報

• CRID: 1390001204171196032
• NII論文ID: 110003666312
• NII書誌ID: AA00602100
• DOI: 10.1248/cpb.53.1510
• ISSN: 13475223
• ISSN: 00092363
• NDL書誌ID: 7675874
• Web Site: https://obihiro.repo.nii.ac.jp/records/362
• Web Site: http://id.ndl.go.jp/bib/7675874
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7675874
• Web Site: http://www.jstage.jst.go.jp/article/cpb/53/11/53_11_1510/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可