Studies on the nutrition of rice cell culture I. A simple, defined medium for rapid growth in suspension culture :

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Author(s)

    • Ohira Koji
    • Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
    • Ojima Kunihiko
    • Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
    • Fujiwara Akio
    • Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University

Abstract

We investigated the nutrient requirements of rice in liquid culture and developed a revised medium of mineral salts, sucrose, thiamine and 2,4-dichlorophenoxyacetic acid. The following major nutrients at the indicated concentrations were beneficial : NO_3-N (40 mM), NH_4-N (5.0 mM), P (2.0 mM) and K (40 mM). Cobalt, iodine, pyridoxine, nic.otinic acid and m-inositol were not essential and were excluded from the revised R-2 medium. Growth was better with ammonium and nitrate together than with nitrate as the sole nitrogen source. Cell growth in the R-2 medium was superior to that obtained in B5, Heller, Murashige-Skoog and White media.

Journal

  • Plant and cell physiology

    Plant and cell physiology 14(6), 1113-1121, 1973

    Japanese Society of Plant Physiologists

Cited by:  26

Codes

  • NII Article ID (NAID)
    110003716708
  • NII NACSIS-CAT ID (NCID)
    AA0077511X
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • Data Source
    CJPref  NII-ELS  NDL-Digital 
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