Purification and properties of an α-amylase from germinating seeds of Phaseolus vidissimus Ten. :

• Yamasaki,Yoshiki

  Institute for Agricultural and Biological Sciences, Okayama University

• Suzuki,Yukio

  Institute for Agricultural and Biological Sciences, Okayama University

抄録

Most of the α-amylase in germinating Phaseolus vidissimus Ten. seeds is found in the cotyledon. α-Amylase was isolated in a highly purified form. The molecular weight was estimated to be 59,000 from the mobility on SDS-polyacrylamide gel electrophoresis. The enzyme readily hydrolyzed amylopectin, amylose, glycogen and soluble starch, and maltotriose very slightly. At the hydrolysis degree of about 18% of soluble starch, at which the iodine color disappeared, oligosaccharides larger than maltotetraose largely accumulate in the reaction mixture, and glucose, maltose and maltotriose a little.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 20 (3), 553-562,

  Japanese Society of Plant Physiologists

キーワード

• Action on raw starch
• a-Amylase
• Phaseolus vidissimus Ten.
• Sub-strate specificity

詳細情報

• CRID: 1542261570220455424
• NII論文ID: 110003717657
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10767703
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles