ATP-Promoted Sorbitol Transport into Vacuoles Isolated from Apple Fruit :

• Yamaki,shohei

  Fruit Tree Research Station (Ministry of Agriculture, Forestry and Fisheries)

抄録

Sorbitol was transported actively into vacuoles isolated from apple (Malus pumilla Mill. var domestica Schneid.) fruit flesh. The uptake was stimulated up to twofold by the addition of ATP, and the ATP dependent uptake showed a saturation curve as to the substrate concentration. The optimum uptake of sorbitol was pursued in the acidic range of pH 5 to 6. The K_m value for the ATP dependent sorbitol uptake was about 5 mM. Sorbitol uptake was clearly inhibited by PCMB and uncouplers (CCCP and DCCD), and to a lesser extent by orthovanadate, but only slightly by oligomycin. K^+ stimulated sorbitol uptake. Sorbitol was converted to other sugars (glucose) only very slowly when transported across the tonoplast. This suggests that sorbitol is transported into vacuoles by a carrier mediated transport system coupled with H^+-ATPase, localized on the tonoplast. Sucrose uptake into the vacuoles was also enhanced by ATP.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 28 (4), 557-564,

  Japanese Society of Plant Physiologists

キーワード

• Apple
• H^+-ATPase
• Membrane transport
• Sorbitol
• Tonoplast
• Vacuole

詳細情報

• CRID: 1543387470127532032
• NII論文ID: 110003719180
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10769713
• 本文言語コード: en
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  • NDL-Digital
  • CiNii Articles