Electrical Characteristics of the Tonoplast of Chara corallina : A Study Using Permeabilised Cells :

• Tester,Mark

  Botany School

• Beilby,Mary J.

  Botany School:(Present)Biophysics Laboratory, School of Biological Sciences, A12, University of Sydney

• Shimmen,Teruo

  Botany School:(Permanent)Department of Botany, Faculty of Science, University of Tokyo

抄録

Use of permeabilised cells of Chara corallina provides a unique opportunify to study the electrical characteristics of the tonoplast whilst being able to control ionic conditions on the outside of the membrane. Current-voltage (I/V) analysis over wide voltage spans, and admittance measurements at 5 Hz showed that many permeabilised cells had a similar conductance and capacitance to the tonoplast of intact cells. Cells developed two regions of negative-slope conductance upon addition of external Cl^-, which suggests the existence of potential-dependent Cl^- channels in the Chara tonoplast. With Cl^- concentrations similar to those expected in vivo, the resting potential was more sensitive to changes in external K^+ than Cl^-; however, a decrease in external K^+ did not significantly alter the shape of the I/V relation.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 28 (8), 1555-1568,

  Japanese Society of Plant Physiologists

キーワード

• Admittance
• Cl^- channel
• Charophyte
• Current-voltage (I
• V)
• Membrane capacitance
• Voltage clamp

詳細情報

• CRID: 1540291245383732736
• NII論文ID: 110003719299
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10769864
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles