Molecular Size of Wound-Induced 1-Aminocyclopropane-1-carboxylate Synthase from Cucurbita maxima Duch. And Change of Translatable mRNA of the Enzyme after Wounding :

• Nakajima,Nobuyoshi

  Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University

• Nakagawa,Naoki

  Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University

• Imaseki,Hidemasa

  Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University

抄録

Wound-induced 1-aminocyclopropane-1-carboxylate (ACC) synthase was purified by an im-munoaffinity column from wounded mesocarp of winter squash (Cucurbita maxima Duch. cv. Ebisu) fruit, and a specific antibody was raised in rabbit. Translatable mRNA coding for ACC synthase was barely detectable in fresh tissue but clearly increased after wounding. The apparent molecular size of the purified enzyme as estimated by SDS-polyacrylamide gel electrophoresis (PAGE) was about 50 kDa. However, SDS-PAGE fluorograms of in vitro translation product of ACC synthase mRNA and the in vivo labeled enzyme as well as Western blot analysis showed that the subunit size of the enzyme was 58 kDa. The enzyme was partially degraded or processed to a 50 kDa peptide both in vivo and in vitro.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 29 (6), 989-998,

  Japanese Society of Plant Physiologists

キーワード

• 1-Aminocyclopropane-1-carboxylate synthase (ACC synthase)
• Cucurbita maxima Duch
• Limited proteolysis
• Molecular size
• Purification (immunoaffinity column)

詳細情報

• CRID: 1542543045197438080
• NII論文ID: 110003719441
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10770052
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles