Thermotropic Properties of Lipids Extracted from Plasma Membrane and Tonoplast Isolated from Chilling-Sensitive Mung Bean (Vigna radiata [L.] Wilczek) :

• Yoshida,Shizuo

  Institute of Low Temperature Science, Hokkaido University

• Washio,Kenji

  Department of Biology, Faculty of Science, Hokkaido University

• Kenrick,Jannet

  Plant Physiology Unit, CSIRO, Division of Food Research and School of Biological Science, Macquore University

• Orr,Glenda

  Plant Physiology Unit, CSIRO, Division of Food Research and School of Biological Science, Macquore University

抄録

Measurements by differential scanning calorimetry (DSC) were carried out to investigate the thermotropic properties of lipids extracted from plasma membrane and tonoplast which were isolated from the chilling-sensitive mung bean (Vigna radiata [L.] Wilczek). Hydrated micelles of the total lipid fractions extracted from plasma membrane and tonoplast showed phase transitions at 3℃ and 9℃, respectively. DSC measurements on the separated classes of lipids revealed that glucocerebroside, a major glycolipid in these membranes, and a highly saturated phospholipid are involved in the phase transitions of the total lipid fractions. Glucocerebrosides extracted from plasma membrane and tonoplast showed phase transitions with onset temperatures at 38℃ and 35℃, respectively. The total phospholipid fractions from plasma membrane and tonoplast also showed phase transitions at 9℃ and 11℃, respectively. However, the addition of glucocerebroside to the total phospholipid fraction in a proportion close to that found in natural membranes resulted in increases in the phase transition temperatures of 4 to 5℃, suggesting a dominant role of the glycolipid in determining the phase behaviors of the total lipid fractions. From these data, it is suggested that a low-temperature-induced phase transition or phase separation of a high-melting-point glucocerebroside and/or a highly saturated phospholipid in plasma membranes and tonoplasts may participate in the mechanisms involved in chilling injury in plants.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 29 (8), 1411-1416,

  Japanese Society of Plant Physiologists

キーワード

• Chilling injury
• Glucocerebroside
• Phase transition
• Vigna radiata [L.] Wilczek

詳細情報

• CRID: 1542824520174157056
• NII論文ID: 110003719501
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10770126
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles