Rapid Cytoplasmic Alkalization and Dynamics of Intracellular Compartmentation of Inorganic Phosphate during Adaptation against Salt Stress in a Halotolerant Unicellular Green Alga Dunaliella tertiolecta: ^<31>P-Nuclear Magnetic Resonance Study :

• Kuchitsu,Kazuyuki

  Institute of Applied Microbiology, University of Tokyo

• Katsuhara,Maki

  Department of Biology, Faculty of Science, University of Tokyo

• Miyachi,Shigetoh

  Institute of Applied Microbiology, University of Tokyo

抄録

By ^<31gt:P-in vivo nuclear magnetic resonance spectroscopy, two intracellular compartments were detected, and their pH values were estimated in intact cells of a halotolerant unicellular green alga Dunaliella tertiolecta. They were identified as the cytoplasm (pH 7.1) and vacuoles (pH 6.0). Vacuoles were also visualized with a fluorescence-differential interference micro-scope. During the adaptation to the salt stress (NaCl concentration from 0.17 to 1.0 M) where cells rapidly synthesize glycerol as osmoticum, both cytoplasmic and vacuolar pH showed transient increases to about 8 and 6.5, respectively. Subsequently, cytoplasmic inorganic phosphate level, as well as sugar phosphates and terminal phosphate group levels increased. These drastic changes in chemical environment in the cytoplasm including pH and inorganic phosphate concentration are discussed to be key factors for osmoregulation that activate the synthesis and inhibit the breakdown of glycerol.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 30 (3), 407-414,

  Japanese Society of Plant Physiologists

キーワード

• Dunaliella tertiolecta (Chlorophyta)
• Inorganic phosphate (P_i)
• Intracellular pH (cytoplasm,vacuole)
• Osmoregulation
• ^31P-nuclear magnetic resonance (NMR)
• Salt stress

詳細情報

• CRID: 1543668945104297728
• NII論文ID: 110003719599
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10770210
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles