Isolation of Genes for Low-Temperature-Induced Proteins in Rice by a Simple Subtractive Method :

• Aguan,Kripamoy

  Laboratory of Plant Genetic Engineering, Biotechnology Institute, Akita Prefectural College of Agriculture:(Present)Division of Molecular Medicine, Clinical Research Centre, Northwick Park Hospital

• Sugawara,Kazuyuki

  Laboratory of Plant Genetic Engineering, Biotechnology Institute, Akita Prefectural College of Agriculture

• Suzuki,Nobuhiro

  Laboratory of Plant Genetic Engineering, Biotechnology Institute, Akita Prefectural College of Agriculture

• Kusano,Tomonobu

  Laboratory of Plant Genetic Engineering, Biotechnology Institute, Akita Prefectural College of Agriculture

抄録

We have isolated cDNAs that correspond to three distinct low-temperature-induced mRNAs from rice cells using an effective, simple subtraction method. The corresponding genes were designated lip (gene encoding low-temperature-induced protein) -5, -9 and - 19. The expression of these genes was slightly stimulated (lip5 and lip19) or unaffected (lip9) by abscisic acid, unlike the expression of the rab16A-D genes which are readily induced by either abscisic acid or high osmotic stress. Time-course analysis revealed that while the lip5 gene was induced 3 h after temperature down-shift, and the lip9 and lip19 genes were induced 6 h after such a shift.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 32 (8), 1285-1289,

  Japanese Society of Plant Physiologists

キーワード

• Abscisic acid
• cDNA subtraction
• Low temperature
• Oryza sativa

詳細情報

• CRID: 1540854195337262336
• NII論文ID: 110003720050
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10770818
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles