Synthesis of Polysaccharides in Phragmoplasts Isolated from Tobacco BY-2 Cells :

• Kakimoto,Tatsuo

  Department of Biology, Faculty of Science, Osaka University

• Shibaoka,Hiroh

  Department of Biology, Faculty of Science, Osaka University

抄録

Autoradiographic experiments using preparations of isolated phragmoplast obtained from tobacco cultured cells revealed that the radioactivity incorporated into insoluble material from UDP-[^3H]glucose was exclusively present at the cell plate of isolated phragmoplasts. Most of the radioactivity incorporated into isolated phragmoplasts from UDP-[^<14>C]glucose was solubilized by 1,3-β-glucanase and the solubilized radioactivity was associated only with glucose, indicating that most of the radioactivity was incorporated into 1,3-β-glucan. In the presence of high concentrations of unlabeled UDP-glucose, isolated phragmoplasts incorporated radioactivity from UDP-[^3H]xylose. Most of the radioactivity incorporated into insoluble material was present at several sites distributed around the nuclei, while only little was found at the cell plate.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 33 (4), 353-361,

  Japanese Society of Plant Physiologists

キーワード

• Autoradiography
• 1,3 β-Glucan
• Phragmoplast
• Tobacco BY-2 cells
• UDPglucose
• UDP-xylose

詳細情報

• CRID: 1541980095244113792
• NII論文ID: 110003720105
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10770906
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles