Cloning of a Complementary DNA that Encodes an Acidic Chitinase which is Induced by Ethylene and Expression of the Corresponding Gene

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Author(s)

    • ISHIGE Fumiharu
    • Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University
    • MORI Hitoshi
    • Department of Biological Regulation, National Institute for Basic Biology
    • YAMAZAKI Ken'ichi
    • Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University
    • IMASEKI Hidemasa
    • Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University, Department of Biological Regulation, National Institute for Basic Biology

Abstract

A complementary DNA encoding an ethylene-inducible acidic chitinase of azuki bean ( Vigna angularis) was isolated, and its complete nucleotide sequence was determined. The nucleotide and deduced amino-acid sequence were very similar to those of an acidic chitinase from cucumber leaves that had been infected with tobacco necrosis virus. The mRNA for the acidic chitinase was not detected in leaves of azuki bean that had not been treated with ethylene, but it appeared 3 h after initiation of treatment with ethylene and its level gradually increased over a period of 19 h. The mRNA also accumulated in response to salicylate or wounding. The expression of the gene in response to wounding was suppressed by 2,5-norbornadiene, but that in response to salicylate was not affected by this inhibitor.

Journal

  • Plant and cell physiology

    Plant and cell physiology 34(1), 103-111, 1993-01

    Japanese Society of Plant Physiologists

References:  56

Cited by:  8

Codes

  • NII Article ID (NAID)
    110003720236
  • NII NACSIS-CAT ID (NCID)
    AA0077511X
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00320781
  • Data Source
    CJP  CJPref  NII-ELS  NDL-Digital 
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