Cloning of a Complementary DNA that Encodes an Acidic Chitinase which is Induced by Ethylene and Expression of the Corresponding Gene :

• ISHIGE,Fumiharu

  Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University

• MORI,Hitoshi

  Department of Biological Regulation, National Institute for Basic Biology

• YAMAZAKI,Kenichi

  Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University

• IMASEKI,Hidemasa

  Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University:Department of Biological Regulation, National Institute for Basic Biology:To whom correspondence should be addressed at Nagoya University

Abstract

A complementary DNA encoding an ethylene-inducible acidic chitinase of azuki bean ( Vigna angularis) was isolated, and its complete nucleotide sequence was determined. The nucleotide and deduced amino-acid sequence were very similar to those of an acidic chitinase from cucumber leaves that had been infected with tobacco necrosis virus. The mRNA for the acidic chitinase was not detected in leaves of azuki bean that had not been treated with ethylene, but it appeared 3 h after initiation of treatment with ethylene and its level gradually increased over a period of 19 h. The mRNA also accumulated in response to salicylate or wounding. The expression of the gene in response to wounding was suppressed by 2,5-norbornadiene, but that in response to salicylate was not affected by this inhibitor.

Journal

• Plant and cell physiology

  Plant and cell physiology 34 (1), 103-111,

  Japanese Society of Plant Physiologists

Keywords

• Acidic chitinase
• cDNA cloning
• Ethylene
• Gene expression
• Vigna angularis(azuki bean)

Details

• CRID: 1540854195337293440
• NII Article ID: 110003720236
• NII Book ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10771078
• Text Lang: en
• Data Source:
  • NDL-Digital
  • CiNii Articles