Expression in Yeast of a Fusion Gene Composed of the Promoter of a Heat-Shock Gene from Arabidopsis and a Bacterial Gene for β-Glucuronidase :

  • TAKAHASHI,Taku
    Molecular Genetics Research Laboratory, The University of Tokyo:(Present)Laboratory of Plant Molecular Biology, The Rockefeller University
  • YABE,Naoto
    Molecular Genetics Research Laboratory, The University of Tokyo
  • KOMEDA,Yoshibumi
    Molecular Genetics Research Laboratory, The University of Tokyo

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Production of a functional β-glucuronidase (GUS) protein was induced by exposure of exponentially growing yeast cells to heat shock after transformation of the GUS gene under the control of the promoter of the heat-shock gene, HSP18.2, from Arabidopsis. Yeast cyr and bcy mutations appeared to have essentially no effect.

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