Protein Phosphorylation in the Sieve Tubes of Rice Plants :
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- NAKAMURA,Shin-ichi
- Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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- HAYASHI,Hiroaki
- Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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- MORI,Satoshi
- Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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- CHINO,Mitsuo
- Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo
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Proteins and their phosphorylation were examined in rice phloem sap that had been collected by the insect laser technique. Analysis by SDS-PAGE indicated that rice phloem sap contained over 150 proteins. The total protein concentration was 150-200 ng μl^<-1>. Analysis of proteins extracted from leaves, roots and seeds revealed that several major low-molecular-weight proteins were confined to the rice phloem sap. Maintenance of rice plants under stable environmental conditions was associated with a constant complement of proteins in the phloem sap. Phosphorylation and dephosphorylation of proteins were detected in the sieve tubes and occurred in a light dependent manner when [γ-^<32>P]ATP was supplied to leaf blades. In darkness, 17-kDa and 24-kDa proteins were phosphorylated. When plants were transferred to the light, 17-kDa and 21-kDa proteins were phosphorylated. Proteins in the rice phloem sap were also phosphorylated in vitro. The phosphorylation was Ca^<2+>_dependent and was inhibited by a protein kinase inhibitor. These results indicate that a signal-transport system that involves phosphorylation of proteins may exist in rice sieve tubes.
収録刊行物
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- Plant and cell physiology
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Plant and cell physiology 34 (6), 927-933,
Japanese Society of Plant Physiologists
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詳細情報 詳細情報について
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- CRID
- 1541698620267445248
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- NII論文ID
- 110003720346
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- NII書誌ID
- AA0077511X
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- 本文言語コード
- en
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- データソース種別
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- NDL-Digital
- CiNii Articles
