Spectrophotometric and Molecular Properties of Mutated Rice Phytochrome A : ENVIRONMENTAL AND STRESS RESPONSES : GENE STRUCTURE AND EXPRESSION :

• TOMIZAWA,Ken-ichi

  Frontier Research Program, Laboratory of Plant Biological Regulation, RIKEN Institute

• STOCKHAUS,Jorg

  Frontier Research Program, Laboratory of Plant Biological Regulation, RIKEN Institute:The Rockefeller University, Laboratory of Plant Molecular Biology:(Present)Institut fur Entwicklungs und Molekularbiologie der Pflanzen, Heinrich-Heine-Universitat

• CHUA,Nam-Hai

  The Rockegeller University, Laboratory of Plant Molecular Biology

• FURUYA,Masaki

  Frontier Research Program, Laboratory of Plant Biological Regulation, RIKEN Institute:Advanced Research Laboratory, Hitachi Ltd.

抄録

A cDNA (PHYA) for the phytochrome A apoprotein (PHYA) of rice and three mutated sequences (phyA S/A, the first ten serine residues in the N-terminal domain of PHYA were changed to alanine residues; phyA ND, the first 80 N-terminal amino acids were deleted; phyA CD, the amino acids of the C-terminal domain from 689 to 1,128 were deleted) were expressed in yeast, and the wild-type and mutant apophytochromes were allowed to combine in vitro with the chromophore phycocyanobilin (PCB). The PCB-attached product of phyA S/A gave very similar spectrophotometric peaks to the PhA_r, and PhyA_<fr> forms of wild-type product. By contrast, the peak of the product of phyA CD in the P_<fr> form was significantly shifted towards a shorter wavelength, an indication that, whereas the C-terminal domain is not crucial for the PCB attachment, it greatly influences the absorption maximum of PhyA_<fr>. The rate of 50% reversion from PhyA_<fr> to PhA_r, in darkness was 3 h at 27℃ with all of the samples, showing that the S/A and CD mutations did not affect this property. No photoreversibility was detected with the product of phyA ND. Gel-filtration analysis of the wild-type PHYA and the product of phyA S/A showed that the apparent molecular mass of each was 330 kDa, suggesting that both exists as dimers in solution.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 36 (3), 511-516,

  Japanese Society of Plant Physiologists

キーワード

• Apophytochrome
• Chromophore
• Mutation
• Phycocyanobilin
• Phytochrome A(rice)
• Spectral properties(phytochrome)

詳細情報

• CRID: 1540572720360650624
• NII論文ID: 110003720629
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10771596
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles