Isolation and Characterization of a cDNA That Encodes Maize Glutamate Dehydrogenase : GENE STRUCTURE AND EXPRESSION :
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- Sakakibara,Hitoshi
- Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University
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- Fujii,Kazutoshi
- Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University:(Present)Biochemical Research Laboratories, Ezaki Glico Co., Ltd.
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- Sugiyama,Tatsuo
- Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University
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抄録
A full-length cDNA clone, μMGDH1, encoding maize NADH-glutamate dehydrogenase (NADH-GDH) was isolated from a maize root cDNA library. The identity of the cDNA was established by the coincidence of the structure of the purified protein with that inferred from the nucleotide sequence of the cDNA. μMGDH1 had a cDNA insert of 1,638 bp and the open reading frame encoded 411 amino acid residues. The deduced amino acid sequence was similar to putative partial sequences of GDHs from higher plants and to the sequences of GDHs from organisms as diverse as mammals and bacteria. The NH_2-terminal sequence deduced from the open reading frame had a typical structure that is associated with the import of proteins into the mitochondrial matrix. The cDNA hybridized to an RNA of about 1.6 kb. This transcript was more abundant in roots than in leaves and was localized in the bundle sheath cells in leaf tissues. Analysis of genomic DNA by Southern hybridization suggested the existence of gene(s) for another NADH-GDH subunit(s).
収録刊行物
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- Plant and cell physiology
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Plant and cell physiology 36 (5), 789-797,
Japanese Society of Plant Physiologists
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詳細情報
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- CRID
- 1541135670314077952
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- NII論文ID
- 110003720665
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- NII書誌ID
- AA0077511X
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- 本文言語コード
- en
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- データソース種別
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- NDL-Digital
- CiNii Articles