Specific Response of Partially Purified Cell Wall-Bound ATPases to Fungal Suppressor :

• Kiba,Akinori

  Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University

• Toyoda,Kazuhiro

  Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University

• Ichinose,Yuki

  Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University

• Yamada,Tetsuji

  Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University

• Shiraishi,Tomonori

  Laboratory of Plant Pathology and Genetic Engineering, College of Agriculture, Okayama University

抄録

It was found that NTPases were bound to cell walls of pea and cowpea. The suppressor in pycnospore germina-tion fluid of a pea pathogen, Mycosphaerella pinodes, inhibited the ATPase activity in the fraction, which was solubilized from pea cell wall with 0.5% Triton X-100, in a dose-dependent manner, but rather enhanced that from cowpea cell wall even at the concentration of 1 μg ml^<-1>. Inhibition by the suppressor of pea cell wall-bound ATPase was a mixed type of competitive and noncompetitive. Triton X-100 PAGE and active staining of ATPase indicated that both Triton X-100 solubilized fractions con-tained plural molecules that hydrolyze ATP. The M_rs of cell wall-bound ATPases seem to be considerably different from those of plasma membranes, and the number of cell wall-bound ATPase molecules were different between pea and cowpea. The electroeluted fractions corresponding to the bands of active-stained ATPases were also able to hydrolyze NTP and PP_i. The respective electroeluted ATPases also showed the species-specific response to the sup-pressor. These results may confirm our previous concept that putative receptors for the suppressor might tightly bind to cell wall-bound ATPase or that the ATPase might be the receptor itself.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 37 (2), 207-214,

  Japanese Society of Plant Physiologists

キーワード

• Cell wall-bound ATPases-Mycosphaerellapinodes-Pisum sativum L.-Species-specificity-Suppressor-Vigna sinensis Endl.

詳細情報

• CRID: 1541417145290814336
• NII論文ID: 110003720808
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10771828
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles