A Step in the Biosynthesis of Gibberellins that Is Controlled by the Mutation in the Semi-Dwarf Rice Cultivar Tan-Ginbozu :

• Ogawa,Shoko

  Biotechnoiogy ResearchCenter, The University of Tokyo

• Toyomasu,Tomonobu

  Department of APPlied Bioiogical Chemistry, The University of Tokyo

• Yamane,Hisakazu

  Biotechnoiogy ResearchCenter, The University of Tokyo

• Murofushi,Noboru

  Department of APPlied Bioiogical Chemistry, The University of Tokyo

• Ikeda,Ryoichi

  National Agriculture Research Center, Ministry of Agriculture, Forestry and Fishery

• Morimoto,Yasuyuki

  National Agriculture Research Center, Ministry of Agriculture, Forestry and Fishery

• Nishimura,Yasuhiko

  Biotechnoiogy ResearchCenter, The University of Tokyo

• Omori,Toshio

  Biotechnoiogy ResearchCenter, The University of Tokyo

抄録

Genetic analysis and a comparison of endogenous levels of gibberellins between the semi-dwarf rice cultivar Tan-ginbozu and the corresponding normal cultivar Ginbozu have confirmed that Tan-ginbozu is a gibberellin-deficient mutant and that the semi-dwarfism of Tan-ginbozu is controlled by a single recessive gene. A step in the biosynthesis of gibberellins that is blocked by the mutation in Tan-ginbozu had been considered to be the synthesis of ent-kaurene or an earlier step. However, the rate of production of ent-kaurene by Tan-ginbozu was almost the same as that by Ginbozu. By contrast, accumulation of only a small amount of ent-kaurene was detected in Tan-ginbozu, and the amount that accumulated was similar to that in Ginbozu that had been treated with 6.9 × 10^<-8> M uniconazole-P (an effective inhibitor of three oxidative steps in the pathway from ent-kaurene to ent-kaurenoic acid via ent-kaurenol and ent-kaurenal). The height of the treated Ginbozu plants was reduced to the same as that of Tan-ginbozu plants. Resembling Tan-ginbozu plants, Ginbozu plants that had been treated with uniconazole-P responded well to ent-kaurenoic acid and slightly to ent-kaurene and ent-kaurenol. Since the growth-promoting activity of ent-kaurenal in Tan-ginbozu was similar to that of ent-kaurene, our results suggest that the mutation in Tan-ginbozu blocks the three oxidative steps whereby ent-kaurene is converted to ent-kaurenoic acid.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 37 (3), 363-368,

  Japanese Society of Plant Physiologists

キーワード

• Dwarfism-ent-Kaurene-Gas chromatography
• selected ion monitoring-Gibberellin-deficient mutant-Oryza sativa L. cv. Tan-ginbozu-Uniconazole-P.

詳細情報

• CRID: 1541698620267528448
• NII論文ID: 110003720829
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10771857
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles