Suppression by 5-Bromo-2'-deoxyuridine of Transdifferentiation into Tracheary Elements of Isolated Mesophyll Cells of Zinnia elegans :

• Shoji,Yuichi

  Research&Development Center, Tohoku Electric PowerCo.

• Sugiyama,Munetaka

  Biological Institute, Graduate School of Science, Tohoku University

• Komamine,Atsushi

  Biological Institute, Graduate School of Science, Tohoku University:(Present)Department of Chemical and BiologicalSciences, Faculty of Science, Japan Women's University

抄録

5-Bromo-2'-deoxyuridine (BrdU), a thymidine analog, suppressed the transdifferentiation into tracheary elements (TE) of isolated mesophyll cells of Zinnia elegans without affecting cell division. Tracer experiments with [^3H]BrdU indicated that 76% and 24% of the incorporated radioactivity was located in the DNA and the RNA, respectively. Both thymidine and uridine counteracted the inhibitory effect of BrdU on transdifferentiation but thymidine was much more effective than uridine. These results suggest that BrdU might interfere with transdifferentiation via its incorporation into DNA. The timing of effective suppression by BrdU was examined by monitoring the effects of the sequential addition of BrdU and thymidine with an interval of 12 h at various times in culture. Transdifferentiation was most sensitive to BrdU between the 24th and the 36th hour of culture. This result suggests that this window of time is critical for DNA synthesis during the transdifferentiation of isolated mesophyll cells of Zinnia elegans into TE.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 37 (3), 401-403,

  Japanese Society of Plant Physiologists

キーワード

• 5-Bromo-2'-deoxyuridine-Tracheary element-Transdifferentiation-Zinnia elegans.

詳細情報

• CRID: 1542824520174371968
• NII論文ID: 110003720835
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10771863
• 本文言語コード: en
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  • NDL-Digital
  • CiNii Articles