Intra- and Extra-Cellular Localization of "Cytosolic" CuZn-Superoxide Dismutase in Spinach Leaf and Hypocotyl :

• Ogawa,Kenichi

  Department of Botany, Faculty of Science, Kyoto University

• Kanematsu,Sumio

  Department of Food Science and Technology, Minami-Kyushu University

• Asada,Kozi

  The Research Institute for Food Science, Kyoto University

Abstract

Immunogold-electron microscopic analysis of spinach leaves done with the antibody specific for "cytosolic" CuZn-superoxide dismutase (SOD) indicates that SOD is localized in the apoplast, in the nucleus and in, or near, the tonoplast. The association of CuZn-SOD with the nucleus indicates it has a role in preventing fatal mutation caused by reactive species of oxygen. The localizing site of CuZn-SOD in the apoplastic region of spinach leaf tissues corresponds to that of the accumulation of lignin. In spinach hypocotyl "cytosolic" CuZn-SOD is localized in vascular tissues where lignification and the generation of superoxide respectively were shown by the phloroglucin-HCI reaction and formation of formazane from nitroblue tetrazolium. Because hydrogen peroxide is required for lignification via the peroxidase-catalyzed reaction, the CuZn-SOD in the apoplast appears to function in the biosynthesis of lignin by causing rapid disproportionation of the superoxide anion radical prior to its interaction with cellular components and peroxidase.

Journal

• Plant and cell physiology

  Plant and cell physiology 37 (6), 790-799,

  Japanese Society of Plant Physiologists

Keywords

• Apoplast-Immuno-gold labeling-Ligninsynthesis-Rapid freezing-Spinach (Spinacia oleracea)-Superoxide dismutase (EC 1.15.1.1).

Details

• CRID: 1542824520174381056
• NII Article ID: 110003720889
• NII Book ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10771941
• Text Lang: en
• Data Source:
  • NDL-Digital
  • CiNii Articles