飼料イネサイレージ調製への乳酸菌 (Lactobacillus plantarum畜草1号)の利用  [in Japanese] Application of Lactic Acid Bacteria (Lactobacillus plantarum Chikuso-1) for Silage Preparation o Forage Paddy Rice.  [in Japanese]

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Abstract

飼料イネサイレージ調製用乳酸菌をスクリーニングするため, ホモ発酵型で耐酸性が強く, 発酵過程において旺盛に増殖できる乳酸菌「畜草1号」菌株を選抜した。 16S rRNA遺伝子の解析やDNA-DNA相同性試験に基づいて畜草1号菌株はLactobacillusplantarumと同定した。畜草1号菌株を添加したはまさりとクサホナミの飼料イネロールベールサイレージでは無添加サイレージに比べ, サイレージのpH値, 酪酸およびアンモニア態窒素含量が低下し, 乳酸含量が高まった。また, 1年間の長期貯蔵を行っても, 糸状菌の増殖がなく, その品質が良質に保持された。

A selected strain Chikuso-1 isolated from corn was characterized, and their application to silage preparation of forage paddy rice was studied. This strain was a Gram- positive and catalase-negative rod that did not produce gas from glucose, formed L (+) and D (-) lactic acid and grew under a low-pH condition. Based on 16S rRNA gene sequence analysis and DNA-DNA relatedness, the strain Chikuso-1 belonged to Lactobacillus plantarum. This strain was used as additives to round bale silage preparation of forage paddy rice with two cultivars, Hamasari and Kusahonami. Silages inoculated with strain Chikuso-1 were well preserved and exhibited lower pH, butyric acid and ammonia-nitrogen, and higher lactic acid content, as compared to control silages. After 370-days of storage, control silages displayed mould growth, whereas in Chikuso-1-inoculated silages, moulds were nearly or below the detectable level. These results showed that Lactobacillus plantarum Chikuso-1 is of great potential as an inoculant for forage paddy rice.

Journal

  • Japanese Journal of Grassland Science

    Japanese Journal of Grassland Science 49(5), 477-485, 2003

    Japanese Society of Grassland Science

References:  32

Cited by:  29

Codes

  • NII Article ID (NAID)
    110003849638
  • NII NACSIS-CAT ID (NCID)
    AN10559677
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    0447-5933
  • NDL Article ID
    6901751
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産) // ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-344
  • Data Source
    CJP  CJPref  NDL  NII-ELS  IR  J-STAGE 
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